Cdc7p-Dbf4p regulates mitotic exit by inhibiting polo kinase

26Citations
Citations of this article
44Readers
Mendeley users who have this article in their library.

Abstract

Cdc7p-Dbf4p is a conserved protein kinase required for the initiation of DNA replication. The Dbf4p regulatory subunit binds Cdc7p and is essential for Cdc7p kinase activation, however, the N-terminal third of Dbf4p is dispensable for its essential replication activities. Here, we define a short N-terminal Dbf4p region that targets Cdc7p-Dbf4p kinase to Cdc5p, the single Polo kinase in budding yeast that regulates mitotic progression and cytokinesis. Dbf4p mediates an interaction with the Polo substrate-binding domain to inhibit its essential role during mitosis. Although Dbf4p does not inhibit Polo kinase activity, it nonetheless inhibits Polo-mediated activation of the mitotic exit network (MEN), presumably by altering Polo substrate targeting. In addition, although dbf4 mutants defective for interaction with Polo transit S-phase normally, they aberrantly segregate chromosomes following nuclear misorientation. Therefore, Cdc7p-Dbf4p prevents inappropriate exit from mitosis by inhibiting Polo kinase and functions in the spindle position checkpoint. © 2009 Miller et al.

Cite

CITATION STYLE

APA

Miller, C. T., Gabrielse, C., Chen, Y. C., & Weinreich, M. (2009). Cdc7p-Dbf4p regulates mitotic exit by inhibiting polo kinase. PLoS Genetics, 5(5). https://doi.org/10.1371/journal.pgen.1000498

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free