Single channel kinetics of a glutamate receptor

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Abstract

The glutamate receptor-channel of locust muscle membrane was studied using the patch-clamp technique. Muscles were pretreated with concanavalin A to block receptor-channel desensitization, thus facilitating analysis of receptor-channel gating kinetics. Single channel kinetics were analyzed to aid in identification of the molecular basis of channel gating. Channel dwell-time distributions and dwell-time autocorrelation functions were calculated from single channel data recorded in the precence of 10–4M glutamate. Analysis of the dwell time distributions in terms of mixtures of exponential functions revealed there to be at least three open states of the receptor-channel and at least four closed states. Autocorrelation function analysis showed there to be at least three pathways linking the open states with the closed. This results in a minimal scheme for gating of the glutamate receptor-channel, which is suggestive of allosteric models of receptor-channel gating. © 1987, The Biophysical Society. All rights reserved.

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APA

Kerry, C. J., Kits, K. S., Ramsey, R. L., Sansom, M. S., & Usherwood, P. N. (1987). Single channel kinetics of a glutamate receptor. Biophysical Journal, 51(1), 137–144. https://doi.org/10.1016/S0006-3495(87)83318-0

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