Long intergenic noncoding RNAs (lincRNAs) have emerged as critical participators in gene regulation in myriads of cell types. The development of the whole transcriptome sequencing technology, or RNA-seq, has enabled novel lincRNA detection, but the bioinformatics analysis toward distinguishing reliable ones remains a challenge. Here, we describe the bioinformatics workflow developed for identifying novel lincRNAs step by step, including read alignment, transcriptome assembly and transcript filtering.
CITATION STYLE
Peng, X., Sun, K., Zhou, J., Sun, H., & Wang, H. (2017). Bioinformatics for novel long intergenic noncoding RNA (lincRNA) identification in skeletal muscle cells. In Methods in Molecular Biology (Vol. 1556, pp. 355–362). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6771-1_20
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