Phytochemical screening, GC-MS, FT-IR analysis of methanolic extract of Curcuma caesia roxb (black turmeric)

Abstract

Aims: The purpose of the current study is to monitor the phytochemical constituents in the Curcuma caesia Roxb.by GC MS and FT-IR analysis. Methods: The Rhizomes of Curcuma caesia Roxb was extracted with Methanol at room temperature for 8 h. The bioactive compounds of Curcuma caesia Roxb have been evaluated using GC-MS and FT-IR. Results: Preliminary phytochemical analysis revealed the presence of tannins, terpenoids, flavonoid, alkaloid, phenol, phytosterol Quinones and saponins. Totally 15 compounds were identified and the chromatograph showed peaks with individual compounds. The major constituents were identified in the Methanolic extract were α-Santalol (46.90%), Retinal (10.72%), Ar-Tumerone(10.38%), Alloaromadendrene (5.93%), Megastigma-3,7(E),9-Triene (4.80%), Benzene, 1-(1,5-dimethyl-4-hexenyl)-4-methyl(4.38%) , 5,8,11,14,17-Eicosapentaenoic acid, methyl ester, (all-Z)-(4.26%) Tricyclo[8.6.0.0(2,9)]hexadeca-3,15-diene, trans-2,9-Anti-9,10-Trans-1,10 (3.26%) and many other compounds were identified as low level. The FTIR analysis confirmed the presence of N-H , O-H , C=C , C-H, C-O and CH3 functional groups. Conclusion: The result of this study offer a platform of using Curcuma caesia Roxb as herbal alternative for various diseases and it can be used as functional and pharmaceutical food.