17- β -estradiol counteracts the effects of high frequency electromagnetic fields on trophoblastic connexins and integrins

7Citations
Citations of this article
16Readers
Mendeley users who have this article in their library.

Abstract

We investigated the effect of high-frequency electromagnetic fields (HF-EMFs) and 17-β-estradiol on connexins (Cxs), integrins (Ints), and estrogen receptor (ER) expression, as well as on ultrastructure of trophoblast-derived HTR-8/SVneo cells. HF-EMF, 17-β-estradiol, and their combination induced an increase of Cx40 and Cx43 mRNA expression. HF-EMF decreased Int alpha1 and β1 mRNA levels but enhanced Int alpha5 mRNA expression. All the Ints mRNA expressions were increased by 17-β-estradiol and exposure to both stimuli. ER-β mRNA was reduced by HF-EMF but augmented by 17-β-estradiol alone or with HF-EMF. ER-β immunofluorescence showed a cytoplasmic localization in sham and HF-EMF exposed cells which became nuclear after treatment with hormone or both stimuli. Electron microscopy evidenced a loss of cellular contact in exposed cells which appeared counteracted by 17-β-estradiol. We demonstrate that 17-β-estradiol modulates Cxs and Ints as well as ER-β expression induced by HF-EMF, suggesting an influence of both stimuli on trophoblast differentiation and migration. © 2013 Franco Cervellati et al.

Cite

CITATION STYLE

APA

Cervellati, F., Valacchi, G., Lunghi, L., Fabbri, E., Valbonesi, P., Marci, R., … Vesce, F. (2013). 17- β -estradiol counteracts the effects of high frequency electromagnetic fields on trophoblastic connexins and integrins. Oxidative Medicine and Cellular Longevity. https://doi.org/10.1155/2013/280850

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free