Comparative genomic of biosynthetic gene cluster of andrimid antibiotic from Serratia plymuthica UBCF_13

Abstract

Serratia plymuthica UBCF_13 was a promising bacterium to control plant pathogenic fungal. Availability of this bacterium genome and genome mining approaches has assisted to identify gene-encoded antibiotics. In this study, we identified the biosynthetic gene cluster (BGC) of andrimid and compared it with andrimid BGC from other bacterial strains and species. Andrimid is a hybrid non-ribosomal peptide-polyketide antibiotic that blocks the carboxyl-transfer reaction of bacterial acetyl-CoA carboxylase (ACC) and thereby inhibits fatty acid biosynthesis with submicromolar potency. Andrimid encoded by a gene cluster containing 20 genes, AdmA-T. Understanding the mechanism of synthesis of the non-ribosomal peptide (NRP) and polyketide assembly will assist the effort to manipulate production and increase efficacy of this antibiotic.