We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale. © 2011 by the Biophysical Society.
Dyksterhuis, L. D., White, J. F., Hickey, M., Kirby, N., Mudie, S., Hawley, A., … Ramshaw, J. A. M. (2011). Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule. Biophysical Journal, 100(9), 2077–2083. https://doi.org/10.1016/j.bpj.2011.03.020