Roles of Mg2+ in TPP-dependent riboswitch

62Citations
Citations of this article
48Readers
Mendeley users who have this article in their library.

Abstract

We quantified the effect of Mg2+ on thiamine pyrophosphate (TPP) binding to TPP-dependent thiA riboswitch RNA. The association constant of TPP binding to the riboswitch at 20°C increased from 1.2 × 106 to 50 × 106 M-1 as the Mg2+ concentration increased from 0 to 1 mM. Furthermore, circular dichroic spectra under various conditions showed that 1 mM Mg2+ induced a local structural change of the riboswitch, which might be pivotal for TPP binding. These results indicate that a physiological concentration of Mg2+ can regulate TPP binding to the thiA riboswitch. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Cite

CITATION STYLE

APA

Yamauchi, T., Miyoshi, D., Kubodera, T., Nishimura, A., Nakai, S., & Sugimoto, N. (2005). Roles of Mg2+ in TPP-dependent riboswitch. FEBS Letters, 579(12), 2583–2588. https://doi.org/10.1016/j.febslet.2005.03.074

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free