In vitro testing of basal cytotoxicity: Establishment of an adverse outcome pathway from chemical insult to cell death

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Abstract

In this paper, an in vitro basal cytotoxicity testing strategy is described for new chemical entities that lack any pre-existing information on potential toxicity. Special attention is paid to the selection of the cellular system, cytotoxicity assay and exposure conditions. This approach is based on a newly proposed generic adverse outcome pathway from chemical insult to cell death that consists of 3 steps, including initial cell injury, mitochondrial dysfunction and cell demise. The suggested strategy to consider in vitro basal cytotoxicity as a first step in evaluating the toxicity of new chemical entities can be placed in a tiered strategy that could be continued by evaluating more specific types of toxicity.

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Vinken, M., & Blaauboer, B. J. (2017, March 1). In vitro testing of basal cytotoxicity: Establishment of an adverse outcome pathway from chemical insult to cell death. Toxicology in Vitro. Elsevier Ltd. https://doi.org/10.1016/j.tiv.2016.12.004

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