Synthetic Lipid Membrane Channels formed by Designed DNA Nanostructures

  • Langecker M
  • Arnaut V
  • Martin T
  • et al.
Citations of this article
Mendeley users who have this article in their library.


We created nanometer-scale transmembrane channels in lipid bilayers by means of self-assembled DNA-based nanostructures. Scaffolded DNA origami was used to create a stem that penetrated and spanned a lipid membrane, as well as a barrel-shaped cap that adhered to the membrane, in part via 26 cholesterol moieties. In single-channel electrophysiological measurements, we found similarities to the response of natural ion channels, such as conductances on the order of 1 nanosiemens and channel gating. More pronounced gating was seen for mutations in which a single DNA strand of the stem protruded into the channel. Single-molecule translocation experiments show that the synthetic channels can be used to discriminate single DNA molecules




Langecker, M., Arnaut, V., Martin, T. G., List, J., Renner, S., Mayer, M., … Simmel, F. C. (2013). Synthetic Lipid Membrane Channels formed by Designed DNA Nanostructures. Biophysical Journal, 104(2), 545a.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free