Synthetic Lipid Membrane Channels formed by Designed DNA Nanostructures

  • Langecker M
  • Arnaut V
  • Martin T
  • et al.
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Abstract

We created nanometer-scale transmembrane channels in lipid bilayers by means of self-assembled DNA-based nanostructures. Scaffolded DNA origami was used to create a stem that penetrated and spanned a lipid membrane, as well as a barrel-shaped cap that adhered to the membrane, in part via 26 cholesterol moieties. In single-channel electrophysiological measurements, we found similarities to the response of natural ion channels, such as conductances on the order of 1 nanosiemens and channel gating. More pronounced gating was seen for mutations in which a single DNA strand of the stem protruded into the channel. Single-molecule translocation experiments show that the synthetic channels can be used to discriminate single DNA molecules

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Langecker, M., Arnaut, V., Martin, T. G., List, J., Renner, S., Mayer, M., … Simmel, F. C. (2013). Synthetic Lipid Membrane Channels formed by Designed DNA Nanostructures. Biophysical Journal, 104(2), 545a. https://doi.org/10.1016/j.bpj.2012.11.3022

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