One of the most promising approaches to combatting multiple drug resistance is based on the use of recombinant endolysins. In this work we study physical, chemical and lytic properties of phage KPP10, a recombinant endolysin effective against Pseudomonas aeruginosa at concentrations of 3 μg/ml. Fused with a fragment of positively charged SMAP-29 peptide into one reading frame, artilysin KPP10 is effective against laboratory strains and clinical isolates of Pseudomonas aeruginosa in the absence of permeabilizers. Our findings encourage preclinical trials of this artilysin in infectious models.
Antonova, N. P., Balabanyan, V. Y., Tkachuk, A. P., Makarov, V. V., & Gushchin, V. A. (2018). Physical and chemical properties of recombinant KPP10 phage lysins and their antimicrobial activity against Pseudomonas aeruginosa. Bulletin of Russian State Medical University, 7(1), 22–29. https://doi.org/10.24075/brsmu.2018.010