Comparative Analysis of Solasodine from in vitro and in vivo Cultures of Solanum nigrum Linn.

Abstract

An efficient protocol was devised for rapid callus induction of Solanum nigrum Linn. from young leaves. MS medium supplemented with different concentrations IAA (1-3 mg/l) with BAP (0.5 mg/l) and NAA (1-3 mg/l) with BAP (0.5 mg/l) for callus initiation. The growth of the calli derived from leaves increased with time of incubation and remained almost constant after 30 days. For solasodine estimation, the field grown plant part of young leaves and in vitro callus (0.5 g each) were weighed and extracted thrice with methanol and subjected to HPLC. The solasodine content of field grown leaves extracts was 0.0798 mg g-1 whereas the solasodine content in the in vitro callus extracts were 0.142 mg g-1 in 2.5 mgL-1 IAA + 0.5 mgL-1 BAP, followed by 0.1162 mg g-1 in 2 mgL-1 NAA + 0.5 mgL-1 BAP. Key words: Callus induction; Solasodine; Solanum nigrum; medicinal plant DOI: 10.3126/kuset.v5i1.2850 Kathmandu University Journal of Science, Engineering and Technology Vol.5, No.1, January 2009, pp 99-103