Mechanism of regulation of PPARG expression of mesenchymal stem cells by osteogenesis-mimicking extracellular matrices

Abstract

The mechanism of regulation of PPARG expression during the osteogenesis of human mesenchymal stem cells (MSCs) was investigated on an extracellular matrix (ECM) model that mimicked the stepwise osteogenesis ECM. Three matrices that mimicked the ECM of MSCs (stem cell matrices), the early stage ECM (early stage matrices), and the late stage ECM (late stage matrices) of osteogenesis were prepared and compared. The matrices showed different effects on the Wnt/β-catenin signal. The β-catenin signal was activated by endogenous Wnt through interaction with chondroitin sulfate chains to suppress PPARG expression on the stem cell matrices and early stage matrices but not on late stage matrices.