NADH-supported generation of H2O2 by permeabilized rat heart mitochondria was partially prevented by the specific complex I-directed inhibitor, NADH-OH, and was significantly stimulated by ammonium. Ammonium did not affect H2O2 production by complex I in coupled submitochondrial particles. The soluble mitochondrial matrix protein fraction catalyzed NADH-dependent H2O2 production, which was greatly (∼10-fold) stimulated by ammonium. We conclude that complex I is not the major contributor to mitochondrial superoxide (hydrogen peroxide) generation and that there are specific ammonium-sensitive NADH:oxygen oxidoreductase(s) in the mitochondrial matrix which are responsible for mitochondrial H2O2 production. © 2008 Federation of European Biochemical Societies.
Grivennikova, V. G., Cecchini, G., & Vinogradov, A. D. (2008). Ammonium-dependent hydrogen peroxide production by mitochondria. FEBS Letters, 582(18), 2719–2724. https://doi.org/10.1016/j.febslet.2008.06.054