Ammonium-dependent hydrogen peroxide production by mitochondria

Citations of this article
Mendeley users who have this article in their library.


NADH-supported generation of H2O2 by permeabilized rat heart mitochondria was partially prevented by the specific complex I-directed inhibitor, NADH-OH, and was significantly stimulated by ammonium. Ammonium did not affect H2O2 production by complex I in coupled submitochondrial particles. The soluble mitochondrial matrix protein fraction catalyzed NADH-dependent H2O2 production, which was greatly (∼10-fold) stimulated by ammonium. We conclude that complex I is not the major contributor to mitochondrial superoxide (hydrogen peroxide) generation and that there are specific ammonium-sensitive NADH:oxygen oxidoreductase(s) in the mitochondrial matrix which are responsible for mitochondrial H2O2 production. © 2008 Federation of European Biochemical Societies.




Grivennikova, V. G., Cecchini, G., & Vinogradov, A. D. (2008). Ammonium-dependent hydrogen peroxide production by mitochondria. FEBS Letters, 582(18), 2719–2724.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free