Identification of Proteolytic Bacterial Isolates in Sediment Ecosystem of Gunung Anyar Mangrove Forest, Surabaya

Abstract

Mangrove ecosystem is a system consisting of biotic and abiotic environments that interact with each other in a mangrove habitat. There are also biological aspects in mangroves, which are influenced by litter production, decomposition, mineral extraction by plants, and other biological activities of all biota in mangrove forests. Litter is an organic material that undergoes several stages of the decomposition process, producing substances that are important for life and productivity of waters, especially in food chain events. Litter is leaves, twigs, fruit, and other parts of mangrove trees that fall. The process of decomposing organic material in ponds involves multiple species of bacteria. These bacteria are composed of various types of bacteria, such as proteolytic, cellulolytic, amylolytic, nitrifying and denitrification bacteria. Organic waste with high protein content will decompose into simpler compounds with the help of proteolytic bacteria. Naturally, proteolytic bacteria are already present in pond waters even with limited quantity and quality. Therefore, it is necessary to have a mediating bacterial inoculum which contains proteolytic bacteria with high ability to tackle organic waste in ponds. This research aims to obtain information on proteolytic bacteria species in the mangrove ecosystem of Gunung Anyar, Surabaya. This is a descriptive research with sampling and data collection. The research activity consists of station research continued with isolation, enzymatic testing, bacterial identification and data analysis. The results show that out of four mangrove sediment samples from Gunung Anyar, Surabaya, two species of bacteria was obtained, namely Yersinia enterocolotica and Enterobacter agglomerans.