Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC

Abstract

Objective: To develop a reserved-phase HPLC method for the determination of praeruptorin A, praeruptorin B, qianhucoumarin E in roots of Peucedanum praeruptorum. Method: Agilent TC-C18 column (4.6 mm x 250 mm, 5 μm) was used at 30°C with the mobile phase of methanol-water (75:25). The flow rate was set at 0.8 mL·min-1. The detection wavelength was 321 nm. Result: The linear response ranged from 3.20-28.80 μg for ± praeruptorin A (r = 0.999 9, n = 5), 1.60-14.40 g for praeruptorin B (r = 0.999 5, n = 5) and 1.64-14.76 g for qianhucoumarin E (r = 0.999 4, n = 5), respectively. Recoveries were 98.92% with RSD 1.6% for praeruptorin A, 99.66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhucoumarin E. Conclusion: The method is quick, simple and repeatable for determination of three coumarin constituents in root of P. praeruptorum.