Effect of luteolin on lipid peroxidation and antioxidants in acute and chronic periods of isoproterenol induced myocardial infarction in rats

Abstract

Aim: The present study was designed to investigate the preventive effect of luteolin on lipid peroxidation, antioxidants and histopathological findings in acute and chronic periods after isoproterenol (ISO)-induced myocardial-infarction (MI) in male Wistar rats. Methods: Luteolin supplemented by intragastric intubation at a daily dose of 0.3 mg/kg body weight in acute and chronic periods following MI. In acute MI model, luteolin had been administered once per day to rat groups during 30 days. On days 29 and 30th the rats of the acute MI control groups were administered 85mg/kg body weight, isoproterenol, intraperitoneally intravel of 24h. In chronic MI model luteolin administered of rat group during 30 days, and on the 1st and 2nd days, the rats of the chronic MI control and luteolin treatment groups were administered ISO by the same way. Results: The ISO-induced rats both in acute and chronic models showed significant increase in the levels of thiobarbituric acid reactive substances, lipid hydroperoxides in the heart and erythrocyte, and significant decrease in the activities of heart and erythrocytes superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione. Oral treatment with luteolin at a daily dose of (0.3 mg/kg b.wt) in both acute and chronic models showed significant decrease in the levels of heart and erythrocyte lipid peroxidation by products and significant increase in the levels of antioxidant system. The protective role of luteolin (0.3 mg/kg b.wt) on ISO-induced myocardial infarction was further confirmed by histopathological examination. Conclusion: Thus, the experiment clearly showed that luteolin ameliorates cardiac damage in ISO-induced myocardial infarction by prevented the accumulation of lipids due to the anti-lipid peroxidative effect. © 2012.