Proteome-wide discovery of in vivo metacaspase substrates can be obtained by positional proteomics approaches such as N-terminal COFRADIC, for example by comparing the N-terminal proteomes (or N-terminomes) of wild-type plants to transgenic plants not expressing a given metacaspase. In this chapter we describe a protocol for the preparation of plant tissue proteomes, including differential isotopic labelling allowing for a comparison of in vivo N-terminomes that serves as the starting point for N-terminal COFRADIC studies.
Tsiatsiani, L., Stael, S., Van Damme, P., Van Breusegem, F., & Gevaert, K. (2014). Caspases, Paracaspases, and Metacaspases: Methods and Protocols. Methods in Molecular Biology (Clifton, N.J.), 1133(January 2014), 255–61. Retrieved from http://link.springer.com/10.1007/978-1-4939-0357-3%0Ahttp://www.ncbi.nlm.nih.gov/pubmed/24567107