Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux

437Citations
Citations of this article
419Readers
Mendeley users who have this article in their library.

Abstract

In plants, cell polarity and tissue patterning are connected by intercellular flow of the phytohormone auxin, whose directional signaling depends on polar subcellular localization of PIN auxin transport proteins. The mechanism of polar targeting of PINs or other cargos in plants is largely unidentified, with the PINOID kinase being the only known molecular component. Here, we identify PP2A phosphatase as an important regulator of PIN apical-basal targeting and auxin distribution. Genetic analysis, localization, and phosphorylation studies demonstrate that PP2A and PINOID both partially colocalize with PINs and act antagonistically on the phosphorylation state of their central hydrophilic loop, hence mediating PIN apical-basal polar targeting. Thus, in plants, polar sorting by the reversible phosphorylation of cargos allows for their conditional delivery to specific intracellular destinations. In the case of PIN proteins, this mechanism enables switches in the direction of intercellular auxin fluxes, which mediate differential growth, tissue patterning, and organogenesis. © 2007 Elsevier Inc. All rights reserved.

Author supplied keywords

Cite

CITATION STYLE

APA

Michniewicz, M., Zago, M. K., Abas, L., Weijers, D., Schweighofer, A., Meskiene, I., … Friml, J. (2007). Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux. Cell, 130(6), 1044–1056. https://doi.org/10.1016/j.cell.2007.07.033

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free