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Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes

Journal of Clinical Microbiology (1990) 28(6) 1465-1468
DOI: 10.1128/jcm.28.6.1465-1468.1990
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Abstract

Enterotoxigenic Escherichia coli (ETEC) strains were readily identified in pure and mixed cultures with nonradioactive, digoxigenin-labeled DNA probes for heat-labile (LTI) and heat-stable (STaI, STaII, and STb) enterotoxins. Digoxigenin-labeled ETEC fragments were more sensitive than and exhibited less nonspecific background contamination than biotinylated ETEC probes.

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Riley, L. K., & Caffrey, C. J. (1990). Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes. Journal of Clinical Microbiology, 28(6), 1465–1468. https://doi.org/10.1128/jcm.28.6.1465-1468.1990

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