ISOLATION, PHYTOCHEMICAL INVESTIGATION AND IN-VITRO ANTIBACTERIAL ACTIVITY OF ETANOLIC EXTRACT USING FABIANA IMBRICATA L.

Abstract

Fabiana imbricata is a medicinal plant with therapeutic potential, belongs to the family Solanaceae also known as, Made mustard and is known for its anti-bacterial activity. I have been selected groups were divided into positive control, negative control, test groupsT1(20µg/ml), T2(40µg/ml),  T3(60µg/ml),  T4(80µg/ml) and T5(100µg/ml),standard group ciprofloxacin 2mg/ml and also prepared the stock solutions and the same way prepared the cultured medium by using the formula from the cultured medium taken and prepared sub cultured medium by using microorganisms. The result of antibacterial activity suggested that effects ofFabiana imbricata  plant extract were tested on phytochemical, results showed the presence of alkaloids, saponins, Phenolic compounds flavonoids, tannins as chemical constituents may have anti-bacterial activity, which is not evaluated till now.Among that result, shows alkaloids and flavonoids were most effective compounds for the antibacterial activity in higher plants. Apart from that saponins show the most responsible and effectively compound for antibacterial activity of Fabiana imbricata. In this study, two solvents were taken ethanolic whole plant extracts from Fabiana imbricata have been tested for Anti-bacterial activity.Fabiana Imbricata was a traditional medicine. We found strong anti-bacterial activity specifically in the ethanol extracts of Fabiana Imbricata. Plant phenolic compounds have been found to possess potent anti- bacterial activity. These flavonoids have been found to possess anti-bacterial properties in various studies. Strong presence of tannins in all the solvent extracts may explain its potent bioactivities are known to possess potent an anti- bacterial properties. The Saponins have already shown as anti- bacterial activity.