Mikroorganisma antagonis sebagai kawalan biologi penyakit utama padi

Abstract

In present study, 26 microbes consisted of 11 fungal isolates and 16 bacterial isolates were screened against blast disease pathogen (Pyricularia oryzae). All isolates were screened in vitro via dual culture bioassay. All fungal isolates collected were isolated from aerobic rice soils and the endophyic bacteria were isolated from the stem of healthy rice plants. Five isolates have been identified to be potential biocontrol agents as they recorded high PIRG (percentage inhibition of radial growth) values of more than 80%. Two isolates were identified as Trichoderma (F15 and F16) while the rest of them were bacteria isolates (I5, I6 and I16). 16S rDNA sequence analysis results showed that all three bacterial isolates were 100% similar to Paenibacillus polymyxa (Gene Bank assession number: GU332610.1).