Genetic bases of tomatо marker-assisted selection in Belarus

Abstract

To develop tomato hybrids and varieties with a high fruit quality, we gradually solved the following tasks: development of DNA-marking methods for long shelf life genes; the genes modifying the biosynthesis of carotenoids and their composition; testing of the elaborated methods on the developed breeding material; the selection of samples with different allelic composition of fruit quality genes; the development of F 1 hybrids using the method of successive crosses and their study; the selection of tomato forms by DNA-typing methods with target genes in F 2 populations to develop valuable breeding samples; the study of carotenoids’ accumulation peculiarities and their inheritance. We used DNA-identification methods for fruit quality genes: nor, rin , nor A (long shelf life), B, og c , hp2 dg , gf-3 (carotenoid content). The tomato hybrids, combining two pigment content genes and one long shelf life gene and the model forms with different combinations of fruit quality genes ( B/rin/gf-3, B/rin/hp2 dg ; B / nor/gf-3, B / nor/hp2 dg ; оg c / rin/gf-3, оg c / rin/hp2 dg ; оg c / nor/gf-3, оg c / nor/hp2 dg ) in a homozygous state were developed. Use of the developed accessions with carotenoid content genes ( og c /hp2 dg , B/hp2 dg ) as maternal forms and the accessions with complex fruit quality genes ( og c /hp2 dg /nor, og c /hp2 dg /rin, B/hp2 dg /nor , B/hp2 dg /rin, og c /gf-3/nor , og c /gf-3/rin , B/gf-3/rin, B/gf-3/nor ) as paternal forms for hybridization contributes to high accumulation of carotenoids and a lond period of fruit storability.