Scotty: A web tool for designing RNA-Seq experiments to measure differential gene expression

Abstract

Motivation: A common question arises at the beginning of every experiment where RNA-Seq is used to detect differential gene expression between two conditions: How many reads should we sequence?Results: Scotty is an interactive web-based application that assists biologists to design an experiment with an appropriate sample size and read depth to satisfy the user-defined experimental objectives. This design can be based on data available from either pilot samples or publicly available datasets. © 2013 The Author Published by Oxford University Press. All rights reserved.