Predominance of Spirastrella inconstans Protein over Enzymes of Marine Bacteria against MRSA

Abstract

Fisherman community people would have significant value if they used powder form of marine organism for wound healing caused by MRSA. The bioactivity was more prevalent in sponge Spirastrella inconstans compared to other marine organism. The protein was fractioned by ammonium sulfate pptn. method and purified using dialysis. About 1000 μl of dialyzate were loaded on Sephadex G-50 chromatog. column and purified 75 fractions at each percentage of satn. were collected. The fraction no. 3, 4 (40% satn.) and no. 4 (60% satn.) bears max. protein content. Amt. of protein content was estd. in UV-Vis Spectrophotometer, preliminary detection of compd. was carried out by TLC. Two fractions were efficient and their Rf values were found to be similar to that of glutamic acid. The mol. wt. of protein was calcd. as 3 000 Da by SDS-PAGE. Marine bacterial cultures were isolated and enzymes (amylase, DNase, esculinase, lipase, gelatinase and alginase) were produced and lipase bearing max. activity was treated against MRSA. Compared to other marine organism, sponge protein obtained during our period of study was effective. [on SciFinder(R)]