Purification and antioxidant properties of triterpenic acids from blackened jujube (Ziziphus jujuba Mill.) by macroporous resins

Abstract

In order to investigate the purification process and antioxidant activity of triterpenic acids from blackened jujube, the macroporous resin was applied to purify the crude extract from blackened jujube. The adsorption and desorption characterizations of five different macroporous adsorption resins (AB-8, D-101, X-5, HPD-100, S-8) for triterpenic acids of blackened jujube were compared, the optimum purification resins were screened, and the purification parameters were optimized. The antioxidant activity of crude extracts and purified products from blackened jujube was analyzed. The results showed that D-101 resin possessed the best effect on the purification of blackened jujube triterpenic acids. The optimum purification parameters were as follows: sample concentration 25.5 μg/ml, 130 ml of the sample volume was with a flow rate of 2.0 ml/min, eluted with 95% ethanol, and speed flow was 1.0 ml/min. The purity of triterpenic acids was increased by 2.49 times after purification with a recovery rate of (78.58 ± 0.67)%. Furthermore, the IC50 values of hydroxyl radical scavenging capacity from triterpenic acids crude extract and purified substances were 0.900 and 0.850 mg/ml, respectively, and the IC50 values of superoxide anion radical were 0.745 and 0.594 mg/ml, respectively, indicating that the antioxidative capacity of the purified product was stronger than the crude extract. The purified triterpenic acids (PTA) groups at different doses had excellent protective effects on H2O2-induced damage HUVEC cells. Results have revealed that triterpenic acids of blackened jujube have good antioxidant function and utilization and development prospects.