Relationship between proteome changes of Longissimus muscle and intramuscular fat content in finishing pigs fed conjugated linoleic acid

Abstract

The present experiment was conducted to determine proteome changes in Longissimus muscle of finishing pigs fed conjugated linoleic acid (CLA), in association with alteration of intramuscular fat content. Previously, seventy-two DurocLandraceLarge White gilts (approximately 60kg) had been fed maize-soyabean meal-based diets with 0, 125 and 25g CLA/kg diet. The CLA contained 3691mg/g cis-9, trans-11 CLA, 3746mg/g trans-10, cis-12 CLA and 537mg/g other isomers. Six pigs per treatment were slaughtered when they reached a body weight of approximately 100kg. Data published from a previous experiment demonstrated that supplementation with 125 or 25g CLA/kg diet increased intramuscular fat content (P<005). The present study investigated the proteome changes in Longissimus muscle of control and pigs supplemented with 25g CLA/kg diet. CLA significantly influenced the abundance of proteins related to energy metabolism, fatty acid oxidation and synthesis, amino acid metabolism, defence, transport and other miscellaneous processes (P<005). The increase in intramuscular fat content was positively correlated with the increased abundance of carbonic anhydrase 3 and aspartate aminotransferase (P<005). We suggest that the proteome changes in Longissimus muscle contributed to greater intramuscular lipid content in CLA-supplemented pigs. © 2010 The Authors.