In HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-233 was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phosphorylation levels became significantly lower than those of their control. FOXO1 was down-regulated mainly in the cytoplasm, while the nuclear FOXO1 level became relatively high compared to the cytoplasm. As the unphosphorylated active form of FOXO1 increased in the cells, cyclin D1/p21/p27 were up-regulated at either mRNA or protein level. Proliferation of the cells was also greatly inhibited when miR-223 was over-expressed. Therein, our data suggest that miR-223 regulates FOXO1 expression and cell proliferation. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Wu, L., Li, H., Jia, C. Y., Cheng, W., Yu, M., Peng, M., … Wu, X. Z. (2012). MicroRNA-223 regulates FOXO1 expression and cell proliferation. FEBS Letters, 586(7), 1038–1043. https://doi.org/10.1016/j.febslet.2012.02.050