Bacterial support as a biostimulant agent (BPNIII, Azzofor) for marginal soil fertility and stimulating seedlings growth in nursery

Abstract

A nursery is the first preparation step for the reclamation of marginal land, supported by fertile, strong, and adaptable seedling to the environment. Therefore, the role of bacteria as a bio stimulating agent is needed to stimulate the growth of seedling during nursery and to enable their subsequent adaptation to the environment. The purpose of this study is to determine the effectiveness and survival rate of bacteria as a growing medium in marginal soil fertilization as well as the accelerated effect of the vegetative growth of seedling during the nursery. This research used the Completely Randomized Design with 3 treatments and 10 replications. The two biostimulant agents used were BPN III (Burkholderia Metallica, B.anthina, Rhizobium radiobacter, Azotobacter sp., Azospirillum sp.) and AZZOFOR (Enterobacter sp., Lysinibacillus xylanilyticus, Achrimobacter ruhlandii, Enterobacter hormaechei, Comamonas testosterone, Enterobacter cancerogeneus, Pseudomonas mosselii, Leclercia adecarboxylata, Citrobacter youngae, Enterobacter hormaechei, Chyuseobaterium indologenes, Achrimobacter xylosoxidans, and Citrobacter farmer), mixed with BPN III and AZZOFOR. The results showed that the biostimulant agents and rhizobacteria (BPN III + AZZOFOR) were suitable and effective in supporting the fertility of the marginal soil with bacterial population 107 CFU gram soil-1. Furthermore, all bacteria stimulated the vegetative growth of seedling, such as plant height, number of leaves, and branch, monthly during the nursery.