Lan et al. present the long non-coding RNA (lncRNA) landscape of hematopoietic stem cell (HSC) development at single-cell resolution, identifying H19 as a lncRNA pivotal for the endothelial-to-HSC transition in the aorta-gonads-mesonephros region that in trans regulates the promoter de-methylation of several critical hematopoietic transcription factors, including Runx1 and Spi1.
Zhou, J., Xu, J., Zhang, L., Liu, S., Ma, Y., Wen, X., … Lan, Y. (2019). Combined Single-Cell Profiling of lncRNAs and Functional Screening Reveals that H19 Is Pivotal for Embryonic Hematopoietic Stem Cell Development. Cell Stem Cell, 24(2), 285-298.e5. https://doi.org/10.1016/j.stem.2018.11.023