The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include assembly of the 8-hydroxyoctanoic acid side-chain via chain extension of a C4-precursor (4-hydroxybutyrate), and construction of the pyrrothine unit from cysteine via (HolA-D, F-H) prior to intact incorporation into thiomarinol (catalysed by TmlU). A series of thiomarinol-related and other minor metabolites have been isolated from wild-type and mutant strains. The results of these investigations are rationalised in terms of the overall biosynthetic pathway. © 2013 The Royal Society of Chemistry.
Murphy, A. C., Gao, S. S., Han, L. C., Carobene, S., Fukuda, D., Song, Z., … Simpson, T. J. (2014). Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp. SANK 73390. Chemical Science, 5(1), 397–402. https://doi.org/10.1039/c3sc52281d