Identification of histone H3 clipping activity in human embryonic stem cells

23Citations
Citations of this article
41Readers
Mendeley users who have this article in their library.

Abstract

Posttranslational histone modifications are essential features in epigenetic regulatory networks. One of these modifications has remained largely understudied: regulated histone proteolysis. In analogy to the histone H3 clipping during early mouse embryonic stem cell differentiation, we report for the first time that also in human embryonic stem cells this phenomenon takes place in the two different analyzed cell lines. Employing complementary techniques, different cleavage sites could be identified, namely A21, R26 and residue 31. The enzyme responsible for this cleavage is found to be a serine protease. The formation of cleaved H3 follows a considerably variable pattern, depending on the timeframe, culture conditions and culture media applied. Contrary to earlier findings on H3 clipping, our results disconnect the link between declining Oct4 expression and H3 cleavage. © 2014 The Authors.

Cite

CITATION STYLE

APA

Vossaert, L., Meert, P., Scheerlinck, E., Glibert, P., Van Roy, N., Heindryckx, B., … Deforce, D. (2014). Identification of histone H3 clipping activity in human embryonic stem cells. Stem Cell Research, 13(1), 123–134. https://doi.org/10.1016/j.scr.2014.05.002

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free