Permafrost thawing exhibits a greater influence on bacterial richness and community structure than permafrost age in Arctic permafrost soils

Abstract

Global warming accelerates permafrost thawing and changes its microbial community structure, but little is known about how microorganisms in permafrost with different ages respond to thawing. Herein, we disentangled the relative importance of permafrost age (young, medium-aged, old, and ancient, spanning from 50 to 5000 years) and thawing status (active, transitional, and permanently frozen) in shaping bacterial community structure using HiSeq sequencing of the 16S rRNA gene. Our results revealed significant influences of both permafrost thawing and age on bacterial richness. The bacterial richness was significantly higher in the young and thawed permafrost, and the richness increase was mainly observed in Firmicutes, Actinobacteria, Chloroflexi, Deltaproteobacteria, and Alphaproteobacteria. Permafrost thawing led to a gradual change in bacterial community structure and increased contribution of determinism. Permutational analysis of variance demonstrated that thawing significantly changed bacterial community structure at all soil ages, but the community convergence due to permafrost thawing was not observed. Structural equation modeling revealed that permafrost thawing exhibited a greater influence on both bacterial richness and community structure than permafrost age. Our results indicate that microorganisms in permafrost with different ages respond differently to thawing, which eventually leads to distinct bacterial community compositions and different organic carbon decomposition processes in Arctic permafrost.