Functional analysis of transcriptional repressor Otx3/Dmbx1

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Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain sections was confirmed by in situ hybridization. These data suggest that Otx3/Dmbx1 represses Otx2-mediated transcription in the developing brain. We also identified the consensus binding sequence [TAATCCGATTA and TAATCC(N2-4)TAATCC] of Otx3/Dmbx1. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Kimura, K., Miki, T., Shibasaki, T., Zhang, Y., Ogawa, M., Saisho, H., … Seino, S. (2005). Functional analysis of transcriptional repressor Otx3/Dmbx1. FEBS Letters, 579(13), 2926–2932.

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