Journal Article

ProPur™: His-tagged protein purification made easy

  • Brownleader M
Nature Methods (2007) 4(2) i-ii
DOI: 10.1038/nmeth1004
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Abstract

ProPur™ affinity spin columns contain a unique flow regulator, FlowGo, which maximizes the contact time between the target protein and the resin. ProPur columns offer the speed of a spin column with the yields and purities of a gravity column or a batch protocol. They are ideal for rapid small-scale and pilot purifications of His-tagged proteins, method development, expression trials, solubility determination and separation of the proteolytically cleaved His tag from the purified protein.

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Brownleader, M. (2007). ProPurTM: His-tagged protein purification made easy. Nature Methods, 4(2), i–ii. https://doi.org/10.1038/nmeth1004

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