Vitamin B-6 deficiency and level of dietary protein affect hepatic tyrosine aminotransferase activity in cats

Abstract

Total activity [pyridoxal 5'-phosphate (PLP) added in the assay] of hepatic tyrosine aminotransferase (TAT) measured in cats at 0300, 0900, 1500 and 2100h was 10.3 ±1.1, 14.0 ± 0.7, 9.8 ± 1.3 and 11.0 ± 0.7 nkat/g liver, indicating little diurnal variation. Activity after 18 h of food deprivation was 10.0 ± 0.3 nkat/g liver, also not different from cats that were eating ad libitum. These findings support the idea that cats have only limited changes in the activity of hepatic TAT compared with rats. Total TAT activity was measured in cats fed high protein (550 g/kg) and low protein (180 g/kg) diets for 4 wk. Cats fed a high protein diet had activities significantly higher (about twice) than cats fed the low protein diet. Hepatic TAT activity of vitamin B-6-deficient cats (diet without pyridoxine for 9 wk) was compared with cats given the same diet with 8 mg pyridoxine/kg. Total hepatic TAT activity in deficient cats was significantly (P < 0.05) lower per gram soluble or total protein (but not per gram liver) than control cats; holoenzyme activity and percentage of active enzyme in deficient cats were also significantly lower by 75 and 64%, respectively. The apparent K(m) of TAT from cats for tyrosine (2.1 mmol/L)was similar to that for rats (1.9 mmol/L), but higher for PLP in cats (0.16 μmol/L) than rats (0.034 μmol/L). Part of the reason for the higher plasma tyrosine in vitamin B-6-deficient cats than rats is the higher K(m) of TAT for PLP in cats than rats.