Polimorfismos genéticos do fator de crescimento do endotélio vascular na pré-eclâmpsia

Abstract

PURPOSE: To identify genetic polymorphisms of endothelial growth factor (VEGF), positions +936C/T and -2578C/A, in women with pre-eclampsia. METHODS: This was a cross-sectional study conducted on 80 women divided into two groups: pre-eclampsia and control. The sample was characterized using a pre-structured interview and data transcribed from the medical records. DNA extraction, amplification of sequences by the Polymerase Chain Reaction (PCR) with specific primers and polymorphism analysis of Restriction Fragment Length Polymorphism (RFLP) were performed to identify polymorphisms. The statistical analysis was performed in a descriptive manner and using the χ 2 test. The multiple logistic regression model was used to determine the effect of polymorphisms on pre-eclampsia. RESULTS:A higher frequency of the T allele of the VEGF +936C/T polymorphism was observed in patients with pre-eclampsia, but with no significant difference. The presence of allele A of the VEGF -2578C/A was significantly higher in the control group. CONCLUSIONS: No significant association was observed between VEGF +936C/Tpolymorphism and pre-eclampsia. For the VEGF -2578C/A polymorphism a significant difference was observed between thecontrol and pre-eclampsia group, with allele A being the most frequent in the control, suggesting the possibility that carriers of allele A have lower susceptibility to the development of pre-eclampsia.