Allosteric communication between the pyridoxal 5'-phosphate (PLP) and heme sites in the H2S generator human cystathionine β-synthase

Abstract

Background: Long range allosteric communication between the heme and PLP cofactors occurs in human cystathionine β-synthase. Results: Seven mutations in the PLP pocket were studied, including one described in homocystinuric patients. Conclusion: The mutations perturb the heme electronic environment 20 Å away and increase propensity for forming an inactive species. Significance: Bidirectional communication between heme and PLP occurs via an α-helix; its disruption is associated with disease. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.