Author Correction: Nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor (Scientific Reports (2017) DOI: 10.1038/s41598-017-04535-6)

Abstract

In the original version of this Article, the authors neglected to cite a previously-published paper which reports prior in vitro analysis of the transcriptional activity of grs357/s357 mutant line. As a result, in the Discussion section under subheading 'Divergence in GRE-independent target gene transcription in the two mutant lines'. "Divergent transcriptomic responses were observed with DEX suppression of the DSS-induced transcription of the cytokine genes il1β, il8 and il6 and of the metalloproteinase genes mmp-13 and DSS-unchallenged mmp-9 in grs357/s357 mutants, but not in the other ones. Hence, the grs357/s357 line directly reveals GRE-independent GC-GR transcribing activities due to their persistence, while the gr-/- line indirectly confirms their occurrence due to their absence with consequent effects on the phenotype, as discussed in details below." Now reads: "Divergent transcriptomic responses were observed with DEX suppression of the DSS-induced transcription of the cytokine genes il1β, il8 and il6 and of the metalloproteinase genes mmp-13 and DSS-unchallenged mmp-9 in grs357/s357 mutants, but not in the other ones. Transcriptional activity of grs357/s357 mutant line was in vitro analysed by Ziv and co-workers (2013)31 that found lack of Gr genomic activity including transcriptional repression linked to AP1 or NF-kB transcription factors. However, these data are not in conflict with our definition of grs357/s357 as being partially silenced: Gr can regulate gene repression either by binding directly to a GRE and interacting with other transcription factors32 (and this way is lost also in grs357/s357) or by tethering with other DNA binding proteins, a way lost in the gr-/- null line while possibly retained in the grs357/s357 mutant. Hence, the grs357/s357 line directly reveals GRE-independent GC-GR transcribing activities due to their persistence, while the gr-/- line indirectly confirms their occurrence due to their absence with consequent effects on the phenotype, as discussed in details below." Additionally, a supplementary table containing the primers used was omitted from the original version of this Article. These errors have been corrected in the HTML version and the PDF version of the Article.