Diagnostic efficacy of Ziehl-Neelsen method against fluorescent microscopy in detection of acid fast bacilli

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Abstract

Objective: To investigate the application of Ziehl-Neelsen (Z-N) and fluorescent microscopy in detection of acid fast bacilli (AFB). Methods: Duplicate smears were prepared from 260 sputum samples and stained with Z-N and fluorescent staining (FS) methods. The efficiency of both methods in primary diagnosis of tuberculosis were evaluated. Results: The smears were positive for AFB in 15 (5.77%) samples by Z-N staining method and in 16 (6.15%) samples by FS method. The sensitivity and specificity of Z-N staining method against FS method were 93.75% and 100% respectively. Conclusions: Though lesser cost-effective than Z-N, FS method is a more sensitive and better case finding tool in detection of AFB. © 2010 Hainan Medical College.

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Gupta, S., Shenoy, V. P., Bairy, I., & Muralidharan, S. (2010). Diagnostic efficacy of Ziehl-Neelsen method against fluorescent microscopy in detection of acid fast bacilli. Asian Pacific Journal of Tropical Medicine, 3(4), 328–329. https://doi.org/10.1016/S1995-7645(10)60079-X

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