Sustained CPEB-Dependent Local Protein Synthesis Is Required to Stabilize Synaptic Growth for Persistence of Long-Term Facilitation in Aplysia

104Citations
Citations of this article
135Readers
Mendeley users who have this article in their library.

Abstract

The time course of the requirement for local protein synthesis in the stabilization of learning-related synaptic growth and the persistence of long-term memory was examined using Aplysia bifurcated sensory neuron-motor neuron cultures. We find that, following repeated pulses of serotonin (5-HT), the local perfusion of emetine, an inhibitor of protein synthesis, or a TAT-AS oligonucleotide directed against ApCPEB blocks long-term facilitation (LTF) at either 24 or 48 hr and leads to a selective retraction of newly formed sensory neuron varicosities induced by 5-HT. By contrast, later inhibition of local protein synthesis, at 72 hr after 5-HT, has no effect on either synaptic growth or LTF. These results define a specific stabilization phase for the storage of long-term memory during which newly formed varicosities are labile and require sustained CPEB-dependent local protein synthesis to acquire the more stable properties of mature varicosities required for the persistence of LTF. © 2008 Elsevier Inc. All rights reserved.

Author supplied keywords

Cite

CITATION STYLE

APA

Miniaci, M. C., Kim, J. H., Puthanveettil, S. V., Si, K., Zhu, H., Kandel, E. R., & Bailey, C. H. (2008). Sustained CPEB-Dependent Local Protein Synthesis Is Required to Stabilize Synaptic Growth for Persistence of Long-Term Facilitation in Aplysia. Neuron, 59(6), 1024–1036. https://doi.org/10.1016/j.neuron.2008.07.036

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free