Does the S phase have an impact on the accuracy of comparative genomic hybridization profiles in single fibroblasts and human blastomeres?

7Citations
Citations of this article
16Readers
Mendeley users who have this article in their library.

Abstract

Objective To investigate if there is an association between single-cell replicative stage and the segmental chromosome imbalances detected by comparative genomic hybridization (CGH). Design First, 135 fibroblasts from cell-line GM03184 (Coriell) at three cell stages (G0/G1, S, and G2/M) were amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) or Sureplex and blindly analyzed by CGH. Second, 85 human blastomeres at the interphase and the metaphase stages, from 30 donated human cryopreserved embryos, were amplified by Sureplex and analyzed by CGH. Setting Academic center for reproductive medicine. Patient(s) None. Intervention(s) None. Main Outcome Measure(s) Incidence of aneuploidy and segmental imbalances detected at the different cell stages. Result(s) In DOP-PCR amplifications of fibroblasts, an increased incidence of segmental abnormalities was detected in the S phase. In Sureplex amplifications of fibroblasts and blastomeres, no differences were detected between the different cell stages. A significantly increased incidence of structural abnormalities was seen in the aneuploid blastomeres. Conclusion(s) The segmental imbalances detected after Sureplex amplification in 73.3% of the cryopreserved embryos analyzed are mainly nontransitory. They correspond to segmental imbalances present in the cells due to chromosome instability, rather than to replicative DNA segments. © 2014 American Society for Reproductive Medicine, Published by Elsevier Inc.

Cite

CITATION STYLE

APA

Ramos, L., Del Rey, J., Daina, G., Martinez-Passarell, O., Rius, M., Tuñón, D., … Navarro, J. (2014). Does the S phase have an impact on the accuracy of comparative genomic hybridization profiles in single fibroblasts and human blastomeres? Fertility and Sterility, 101(2). https://doi.org/10.1016/j.fertnstert.2013.10.031

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free