Improvement of quality control system of the in vitro production of sugarcane

Abstract

The Sugarcane Biofactory at the Villa Clara Center Sugarcane Research Territorial Station plays a vital role in sugarcane production, since it provides seeds of high genetic and phytosanitary quality obtained by biotechnological methods. For this, strict control of the processes is required, derived from the requirements of environmental and personal asepsis established by in vitro propagation technology. However, the current quality control system presents a group of limitations and insufficiencies related to their information that were not related to each other, which makes it impossible to determine with certainty and speed the causes of production losses. Additionally, it is not complemented with operating procedures of the processes that are carried out in production. This problem was solved with the design and implementation of a group of improvements in the quality control system and operating procedures. These are validated by the results in a group of indicators: 100% customer satisfaction is achieved for the productions delivered, 3.8% lost in vitro production, 95% viable in vitro establishment, 99.3% of culture media produced with quality, 93% survival of in vitro plants during acclimatization, among others. All of this led to 100% compliance with the production plan.