Vitamin E Improves Doxorubicin Induced Nephrotoxicity; Possible Underlying Mechanisms

Abstract

Background: Doxorubicin (Dox) nowadays is a widely used chemotherapy, however using it has a lot of side effects on different body organs. Unfortunately Nephrotoxcisty is one of its common side effects.Aim of Work: In this article we tried to seek for the effect of Vitamin E (Vit E) as a commonly used vitamin to reduce Dox induced nephrotoxisty.Material and Methods: 40 albino rats were involved, they were divided into 4 groups: Control group, Dox group in which Dox was given in a dose of 2.5mg/kg, 3 times/week, Vit E group in this group rats were given Vitamin E alone for 250mg/kg/day daily via intramuscular injection, Dox + Vit E rats in this group received both medications in the previously mentioned doses. At the end of the experimental period blood samples were collected for biochemical analysis of serum Urea, Creatinin and 8-Hydroxy-2´-deoxyguanosine (OHdG). Renal tissues were collected for analysis of tissue OHdG, histological examination (H & E) and immunohistochemical analysis of renal tissue caspase 3 levels.Results: Our results showed deterioration of all parameters and increased immunohistochemical expression of caspase 3 in Dox treated group. On the other hand administration of Dox with Vit E significantly improved all measured parameters and lowers the expression caspase 3 levels.Conclusion: Combined treatment of Dox with Vitamin E decreases risk of Dox induced nephrotoxcisty. Protective effects of Vitamin E can be due its antioxidant and its antia-poptotic actions.