Functional expression studies in microorganisms showed that the Arabidopsis thaliana gene At4g04870 represents the cardiolipin synthase (CLS) gene encoding a hydrophobic preprotein of 38 kDa with a cleavable signal peptide for the import into mitochondria. CLS of Arabidopsis over-expressed in Escherichia coli has an alkaline pH optimum, a strict requirement for divalent cations and a distinctly lower Km for cytidinediphosphate-diacylglycerol than for phosphatidylglycerol. It displayed a preference for both its substrates esterified with unsaturated acyl groups. Solubilization and purification experiments revealed that the protein requires a defined phospholipid environment, particularly the presence of cardiolipin, to acquire its catalytically active conformation. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Nowicki, M., Müller, F., & Frentzen, M. (2005). Cardiolipin synthase of Arabidopsis thaliana. FEBS Letters, 579(10), 2161–2165. https://doi.org/10.1016/j.febslet.2005.03.007