Diversity of Arbuscular Mycorrhizal Fungi as Affected by Time Consequences Revegetation Age in Post Coal Mine Area at PT Berau Coal Tbk, East Kalimantan Indonesia

Abstract

Arbuscular Mycorrhizal Fungi (AMF) is one of the fungi that can be found in almost every ecosystem including post-coal mining land. This study aims to analyze the diversity of AMF in different age of revegetation of post-coal mining land area. Soil samples were collected from six different age of revegetation of post-coal mining area (unrevegetated area, 0, 2, 4, 6 and 8 years) and from natural forest as comparison. AMF spore isolation was carried out using wet-sieving method, while spore identification was carried out based on several morphological characters according to INVAM guideline. The results of the observation showed that with increasing of revegetation age will be able to increase the number of AMF populations (R2= 84.96%). The highest AMF population was found at the age of 10 years after revegetation of land with the number of 492 spores per 20 grams soil, while the lowest AMF population was found in unrevegetated area with the number of 12 spores per 20 grams of soil. AMF genera found included Glomus, Acaulospora, Gigaspora, Scutellospora, and Dentiscutata. Glomus and Acaulospora were the dominant AMF in every land revegetation age (100% of frequency). AMF populations was correlated strongly with soil organic content (r = 0.79).