Uji Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus

  • Tuntun M
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Abstract

Effectiveness of the Leaf Extract of Papaya (Carica papaya L.) on bacterial Growth: Escherichia coli and Staphylococcus aureus. Carica papaya is widely used as a traditional medicine society. Papaya leaves contain antibacterial compounds such as tannins, alkaloids, flavonoids, terpenoids, saponins and alkaloids karpain. Escherichia coli and Staphylococcus aureus are bacterial pathogen that frequently infects in humans. This study was to know ability of the leaf extract of papaya to inhibit the bacterial growth: E. coli and S. aureus and determine the effective inhibition concentration. The study was a laboratory experiment. "Kirby Bauer" diffusion method agar were used to inhibition test. Variable of this study were concentration of the leaf extract of papaya (10% -100%) and the growth inhibition zone of E. coli and S. aureus. Data were analyzed by ANOVA. The results showed that F value > F table, both of bacteria: E. coli and S. aureus. It showed that the leaf extract of papaya (Carica papaya L.) had an influence on the bacteria growth, but did not effective than positive control: Chlorampenicol 30 mcg. Abstrak: Uji Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. Daun pepaya banyak digunakan masyarakat sebagai obat tradisional. Daun pepaya mengandung senyawa antibakteri seperti tanin, alkaloid, flavonoid, terpenoid, saponin, dan alkaloid karpain. Bakteri Escherichia coli dan Stapylococcus aureus merupakan bakteri pathogen yang sering menginfeksi manusia. Tujuan penelitian untuk mengetahui kemampuan ekstrak daun pepaya (Carica papaya L.) dalam menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus dan mengetahui konsentrasi ekstrak daun pepaya (Carica papaya L.) yang efektif dalam menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcu aureus. Jenis penelitian adalah eksperimen laboratorium. Uji daya hambat menggunakan metode difusi agar cara Kirby Bauer. Variabel penelitian yaitu konsentrasi ekstrak daun pepaya 10%-100%, dan zona hambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Analisa data menggunakan uji Anova. Hasil penelitian ini didapatkan F hitung > F tabel, baik terhadap bakteri Escherichia coli maupun bakteri Staphylococcus aureus, hal ini menunjukkan bahwa ekstrak daun pepaya (Carica papaya L.) mempunyai pengaruh terhadap pertumbuhan bakteri tersebut, tetapi tidak efektif jika dibandingkan dengan zona hambat antibiotik Chlorampenicol 30 mcg (kontrol positif) Kata kunci: Daun pepaya, Escherichia coli, Staphylococcus aureus

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APA

Tuntun, M. (2016). Uji Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Kesehatan, 7(3), 497. https://doi.org/10.26630/jk.v7i3.235

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