The influence of metazachlor on vitellogenesis in juvenile (20 days old) zebrafish (Danio rerio) was investigated after ambient water exposure to concentrations of 0.1, 1.0 and 5.0 mg l-1 of the chloroacetanilide herbicide Butisan 400 SC containing approximately 35.6% (w/w) metazachlor. After 20 days of exposure, vitellogenin concentrations in whole-body homogenates of the fish were measured by direct sandwich ELISA. The results were compared to vitellogenin concentrations in fish from both negative (no exposure) and positive (exposed to natural oestrogen 17β-oestradiol) control groups. Exposure to Butisan 400 SC at a concentration of 5.0 mg l-1 induced vitellogenin synthesis significantly compared to the control fish (p < 0.05). The oestrogenic effect of 17β-oestradiol was confirmed.
Jurčíková, J., Mikula, P., Dobšíková, R., Némethová, D., & Svobodová, Z. (2007). Effects of metazachlor on vitellogenin induction in Zebrafish (Danio rerio). Acta Veterinaria Brno, 76(SUPPL. 8). https://doi.org/10.2754/avb200776S8S061