Phytochemistry and Antiperoxidative Potential of Cannabis sativa L. Leaves Methanol Extracts: An In Vitro Study

Abstract

Aim: Present study aimed to demonstrate the preliminary phytochemical composition, antioxidant and in vitro protective potential of the methanolic extract of Cannabis sativa L. Study Design: A methanolic extract of Cannabis sativa L. leaves was prepared and assessed at the in vitro level for its preliminary phytochemical screening and antiperoxidative potentials by using the DPPH radical reduction assay, lipid peroxidation inhibitory activity, protein carbonyl inhibitory potential in glycated bovine serum albumin, and enzyme antioxidant activity in a goat liver homogenate system exposed to hydrogen peroxide as oxidant molecule. Results: The methanolic extract of Cannabis sativa L. leaf contained a diverse range of phytoconstituents, including alkaloids, flavonoids, tannins, terpenoids, and saponins. Upon quantitative analysis, the methanolic extract of  C. sativa L. reflected 50 ± 0.013 µg quercetin equivalent / mg of extract according to the querecetin standard graph, while 28.03 ± 0.024 % alkaloid content was retrieved after quantification. Conclusion: We suggest that, upon tested on all in vitro antioxidant parameters; the leaf methanolic extract of Cannabis sativa L. demonstrated potent antioxidant and protective activity. Keywords: Cannabis sativa L., Antioxidant, in vitro, DPPH, LPO, Protein carbonyl, peroxidative potential