Cytotoxic compounds from the deep-sea sediment-derived Streptomyces malaysiensis OUCMDZ-2167

Abstract

This paper is designed to study the bioactive natural products sourced from the deep-sea sediment-derived Streptomyces malaysiensis OUCMDZ-2167. By means of column chromatography on silica gel, sephadex LH-20 and semi-preparative HPLC, a new furan derivative 1 and ten known compounds 2~11 were isolated and purified from the fermentation broth of S. malaysiensis OUCMDZ-2167. Their structures were respectively elucidated as methyl 4-(hydroxymethyl)furan-2-carboxylate (1), dimethyl furan-2, 4-dicarboxylate (2), geldanamycin (3), 17-O-demethylgeldanamycin (4), nigericin (5), nigericin sodium salt (6), 30-O-acetylnigericin (7), abierixin (8), elaiophylin (9), surugapyrone A (10) and germicidin A (11), by analyzing the MS, UV, IR, NMR and specific rotations. The cytotoxicities against MCF-7, A549 and K562 were evaluated by thiazolyl blue tetrazolium bromide (MTT) and the cell counting kit-8 (CCK-8) methods. The results showed that compounds 3 and 5~9 exhibited the cytotoxicities against A549 cell with the half inhibitory concentrations (IC50) of 2.56±0.30, 0.96± 0.03, 0.96±0.04, 0.81±0.05, 7.11±0.90 and 4.09±0.70 μmol•L-1, respectively. Compounds 5~9 displayed the cytotoxicities against K562 cells with the IC50 values of 1.46±0.11, 1.08±0.03, 0.48±0.10, 4.68±0.19 and 9.60±0.50 μmol • L-1, respectively. And compounds 5, 8 and 9 showed cytotoxicities against MCF-7 cell with the IC50 values of 0.29±0.15, 4.05±0.50 and 4.81±0.70 μmol•L-1, respectively. It is the first time to report the cytotoxicities of compounds 5~8 against K562 and A549 cell lines and against MCF-7 cell line.